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5-alpha-Cholestane is the non-hydroxy, hydrogenated form of cholesterol and is useful as an internal standard for studies involving sterols.1 Cholesterol is a sterol that is essential for all animal life, being critical for membrane permeability and fluidity and for many cellular functions. It is a sterol that is both synthesized in animals and also acquired from the diet. Phytosterols have been found to compete with cholesterol for absorption in the intestinal tract which results in a reduction of cholesterol absorption.2 Plant sterols are important components of membranes and have a particular role in the plasma membrane, mitochondrial outer membrane, and endoplasmic reticulum. Plant sterols will complex with glycosphingolipids in raft-like sub-domains and can affect many cellular functions including membrane fluidity, permeability, activity of membrane-bound enzymes, cellular differentiation, cellular signaling, and cellular proliferation. Plant sterols have been used extensively in humans to attempt to lower cholesterol and treat certain cancers.3
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